Section: New Results

Motion compensation in two-photon microscopy temporal series

Participants : Caroline Medioni, Grégoire Malandain, Florence Besse, Xavier Descombes.

Acquisitions of 3D image sequences over long period of time, in particular, have enabled neurobiologists to follow complex processes such as the development of neuronal populations or degenerative events occurring in pathological contexts, improving our understanding of the mechanisms involved in brain development and function. In most cases, live samples are moving/growing during long-term imaging. Therefore it is required to compensate for this global 3D motion before measuring the dynamics of the structure of interest. We have proposed a method to compute a coherent 3D motion over a whole temporal sequence of 3D volumes (Figure 2 ), which is able to capture subtle sub-voxelic displacements.

Figure 2. Left and middle: mip views of both the first and the last volumes of a temporal series. Right: mip view of the last volume after motion compensation.